Color Swirls of PX458 Transfection

Artist

Alyssa Giaccone

Description

CRISPR is a powerful genomic tool allowing scientists to knock out or add sequences to DNA by use of guide RNAs and Cas nucleases. Guide RNAs target specific sequences ensuring a precise location is affected in the “change” made by CRISPR editing. A Cas nuclease is an enzyme used to snip in or out the desired sequence. A plasmid, PX458, is used to deliver the guide RNA and CAS9 into the cell’s nucleolus where DNA is housed.

A) Blue: DAPY is a fluorescent dye which binds to nucleic acids therefore, identifying the nucleus.

B) Green: GFP gene expressed shows the successful insertion of the plasmid, PX458.

C) Merge of blue and green channels: successful transfection of PX458 suggesting the expression of the gRNA and Cas9.

Type

Microscope

Artist Affiliation

Graduate student

Department

Department of Biological Science

Area of Research

Chemotherapy molecular pathways

Date

2025